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| Antibiotics (Basel, Switzerland). 2023 May 5. doi: 10.3390/antibiotics12050851. pii: antibiotics12050851 |
| Screening of Antibiotic and Virulence Genes from Whole Genome Sequenced Isolated from Food and Milk-Producing Environments. |
| Holý O1, Parra-Flores J2, Bzdil J3, Cabal-Rosel A4, Daza-Prieto B5, Cruz-Córdova A6, Xicohtencatl-Cortes J7, Rodríguez-Martínez R8, Acuña S9, Forsythe S10, Ruppitsch W11 |
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Abstract The objective of this study was to use whole-genome sequencing (WGS) to screen for genes encoding for antibiotic resistance, fitness and virulence in strains that had been isolated from food and powdered-milk-producing environments. Virulence (VGs) and antibiotic-resistance genes (ARGs) were detected with the Comprehensive Antibiotic Resistance Database (CARD) platform, ResFinder and PlasmidFinder tools. Susceptibility testing was performed using disk diffusion. Fifteen presumptive strains of spp. were identified by MALDI-TOF MS and ribosomal-MLST. Nine strains were found in the meningitic pathovar ST4: two were ST83 and one was ST1. The ST4 strains were further distinguished using core genome MLST based on 3678 loci. Almost all (93%) strains were resistant to cephalotin and 33% were resistant to ampicillin. In addition, 20 ARGs, mainly involved in regulatory and efflux antibiotics, were detected. Ninety-nine VGs were detected that encoded for OmpA, siderophores and genes involved in metabolism and stress. The IncFIB (pCTU3) plasmid was detected, and the prevalent mobile genetic elements (MGEs) were ISEsa1, ISEc52 and ISEhe3. The isolates analyzed in this study harbored ARGs and VGs, which could have contributed to their persistence in powdered-milk-producing environments, and increase the risk of infection in susceptible population groups. |
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KEYWORDS: Cronobacter sakazakii, antibiotics resistance, environment, food, virulence genes, whole genome sequencing |
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Publikations ID: 37237754 Quelle: öffnen |
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