Abk�rzung zur Hauptnavigation Abk�rzung zu den Newsmeldungen Abk�rzung zu den Topstories  
  Barrierefreiheit    Kontakt MedUni Wien    Intranet    MedUni Wien - Shop    Universitätsbibliothek    Universitätsklinikum AKH Wien  
 
ccc_logo_en.gif
 
AKH Wien
 
 
Hauptnavigation
  • Livestream 2021
  • Home
  • Über das CCC
    • Allgemeines
    • Leitung der Organisationseinheit
    • CCC-Office Team
    • Kliniken und Partner
    • Qualitätsmanagement
    • Kontakt
  • PatientInnen
    • Covid-19
    • Allgemeines
    • Cancer School
    • Terminvereinbarung
    • Pflegeambulanz
    • PatientInnenvertretung
    • Links
  • Klinischer Bereich
    • Allgemeines
    • CCC Tumorboards
  • Wissenschaft & Forschung
    • Young CCC
    • CCC-ExpertInnenvideos
    • CCC Forschungscluster
    • CCC Units
    • CCC Platforms
    • Translationale Forschung
    • CCC Best Paper Award
    • CCC-TRIO Symposium
    • Kontakt/Links
  • Lehre
    • CCC Cancer School
    • Vienna International Summer School on Clinical and Experimental Oncology - VSSO
    • CCC Excellence Lecture
    • Interdisziplinäre onkologische Ausbildung
    • Klinisch-Praktisches Jahr (KPJ)
    • PhD Programme
    • Postgraduelle Fort- und Weiterbildung
    • Information/Contact
 
 
Subnavigation
    Inhaltsbereich


    Zurück zur Übersicht
    Nature methods. 2022 May 11. doi: 10.1038/s41592-022-01468-5. pii: 10.1038/s41592-022-01468-5
    A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues.
    Glaser AK1,  Bishop KW2,  Barner LA3,  Susaki EA4,  Kubota SI5,  Gao G6,  Serafin RB7,  Balaram P8,  Turschak E9,  Nicovich PR10,  Lai H11,  Lucas LAG12,  Yi Y13,  Nichols EK14,  Huang H15,  Reder NP16,  Wilson JJ17,  Sivakumar R18,  Shamskhou E19,  Stoltzfus CR20,  Wei X21,  Hempton AK22,  Pende M23,  Murawala P24,  Dodt HU25,  Imaizumi T26,  Shendure J27,  Beliveau BJ28,  Gerner MY29,  Xin L30,  Zhao H31,  True LD32,  Reid RC33,  Chandrashekar J34,  Ueda HR35,  Svoboda K36,  Liu JTC37
    Author information
    1Department of Mechanical Engineering, University of Washington, Seattle, WA, USA. akglaser@uw.edu.
    2Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.
    3Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.
    4Department of Biochemistry and Systems Biomedicine, Graduate School of Medicine, Juntendo University, Tokyo, Japan.
    5Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
    6Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.
    7Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.
    8Allen Institute for Brain Science, Seattle, WA, USA.
    9Allen Institute for Brain Science, Seattle, WA, USA.
    10Cajal Neuroscience, Seattle, WA, USA.
    11Leica Microsystems, Inc, Buffalo Grove, IL, USA.
    12Leica Microsystems, Inc, Buffalo Grove, IL, USA.
    13Department of Restorative Sciences, Texas A&M University, Dallas, TX, USA.
    14Department of Genome Sciences, University of Washington, Seattle, WA, USA.
    15Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.
    16Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.
    17Department of Immunology, University of Washington, Seattle, WA, USA.
    18Department of Immunology, University of Washington, Seattle, WA, USA.
    19Department of Immunology, University of Washington, Seattle, WA, USA.
    20Department of Immunology, University of Washington, Seattle, WA, USA.
    21Department of Urology, University of Washington, Seattle, WA, USA.
    22Department of Biology, University of Washington, Seattle, WA, USA.
    23MDI Biological Laboratory, Bar Harbor, ME, USA.
    24MDI Biological Laboratory, Bar Harbor, ME, USA.
    25Department for Bioelectronics, Vienna University of Technology, Vienna, Austria.
    26Department of Biology, University of Washington, Seattle, WA, USA.
    27Department of Genome Sciences, University of Washington, Seattle, WA, USA.
    28Department of Genome Sciences, University of Washington, Seattle, WA, USA.
    29Department of Immunology, University of Washington, Seattle, WA, USA.
    30Department of Urology, University of Washington, Seattle, WA, USA.
    31Department of Restorative Sciences, Texas A&M University, Dallas, TX, USA.
    32Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, USA.
    33Allen Institute for Brain Science, Seattle, WA, USA.
    34Allen Institute for Neural Dynamics, Seattle, WA, USA.
    35Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, Osaka, Japan.
    36Allen Institute for Neural Dynamics, Seattle, WA, USA.
    37Department of Mechanical Engineering, University of Washington, Seattle, WA, USA. jonliu@uw.edu.
    Abstract

    Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a flexible system that can address imaging applications with varied requirements in terms of resolution, sample size, tissue-clearing protocol, and transparent sample-holder material. Here, we present a 'hybrid' system that combines a unique non-orthogonal dual-objective and conventional (orthogonal) open-top light-sheet (OTLS) architecture for versatile multi-scale volumetric imaging. We demonstrate efficient screening and targeted sub-micrometer imaging of sparse axons within an intact, cleared mouse brain. The same system enables high-throughput automated imaging of multiple specimens, as spotlighted by a quantitative multi-scale analysis of brain metastases. Compared with existing academic and commercial light-sheet microscopy systems, our hybrid OTLS system provides a unique combination of versatility and performance necessary to satisfy the diverse requirements of a growing number of cleared-tissue imaging applications.


    © 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.

    Publikations ID: 35545715
    Quelle: öffnen
     
    Drucken
     
    ccc_logo_en.gif
    ccc_logo_en.gif
    ccc_logo_en.gif

    Schnellinfo

     
    -- Initiative Krebsforschung / Krebsforschungslauf

    -- Cancer Care
    -- Kliniken und Partner
    -- CCC Cancer School
    -- Young CCC
    -- CCC Tumorboards
    -- CCC Forschungscluster
    -- CCC Units
    -- CCC Platforms
    -- SOPs / Leitlinien
    -- Kontakt
    Zuklappen
     
    Ausklappen
     
     

    Featured

     
     
     
     
     
     
     
     
     
     
     
     
     
    © MedUni Wien |
     Impressum | Nutzungsbedingungen | Kontakt