Cancers. 2021 Dec 21. pii: cancers14010004. doi: 10.3390/cancers14010004 |
Prognostic Significance of Methylation in Plasma Cell-Free DNA in Advanced High-Grade Serous Ovarian Cancer. |
Tserpeli V1, Stergiopoulou D2, Londra D3, Giannopoulou L4, Buderath P5, Balgkouranidou I6, Xenidis N7, Grech C8, Obermayr E9, Zeillinger R10, Pavlakis K11, Rampias T12, Kakolyris S13, Kasimir-Bauer S14, Lianidou ES15 |
Abstract BACKGROUND: Epigenetic alterations in ctDNA are highly promising as a source of novel potential liquid biopsy biomarkers and comprise a very promising liquid biopsy approach in ovarian cancer, for early diagnosis, prognosis and response to treatment. METHODS: In the present study, we examined the methylation status of six gene promoters (, , , , and in high-grade serous ovarian cancer (HGSOC). We evaluated the prognostic significance of DNA methylation of these six gene promoters in primary tumors (FFPEs) and plasma cfDNA samples from patients with early, advanced and metastatic HGSOC. RESULTS: We report for the first time that the DNA methylation of in plasma cfDNA was significantly correlated with worse PFS ( = 0.045) in advanced stage HGSOC. CONCLUSIONS: Our results strongly indicate that epigenetic inactivation could be a predictor of resistance to platinum drugs in ovarian cancer. Our results should be further validated in studies based on a larger cohort of patients, in order to further explore whether the DNA methylation of promoter could serve as a potential prognostic DNA methylation biomarker and a predictor of resistance to platinum-based chemotherapy in ovarian cancer. |
KEYWORDS: DNA methylation, Schlaffen11, high-grade serous ovarian cancer, liquid biopsy, methylation specific PCR, plasma cell-free DNA, prognostic biomarker, progression free survival |
Publikations ID: 35008168 Quelle: öffnen |