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    Frontiers in microbiology. 2021 Jul 30. doi: 10.3389/fmicb.2021.699858. pmc: PMC8361477
    Production, Storage Stability, and Susceptibility Testing of Reuterin and Its Impact on the Murine Fecal Microbiome and Volatile Organic Compound Profile.
    Castellani C1,  Obermüller B2,  Kienesberger B3,  Singer G4,  Peterbauer C5,  Grabherr R6,  Mayrhofer S7,  Klymiuk I8,  Horvath A9,  Stadlbauer V10,  Russmayer H11,  Miekisch W12,  Fuchs P13,  Till H14,  Heinl S15
    Author information
    1Department of Paediatric and Adolescent Surgery, Medical University of Graz, Graz, Austria.
    2Department of Paediatric and Adolescent Surgery, Medical University of Graz, Graz, Austria.
    3Department of Paediatric and Adolescent Surgery, Medical University of Graz, Graz, Austria.
    4Department of Paediatric and Adolescent Surgery, Medical University of Graz, Graz, Austria.
    5Department of Food Science and Technology, University of Natural Resources and Life Sciences, Vienna, Austria.
    6Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
    7Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
    8Core Facility of Molecular Biology, Medical University of Graz, Graz, Austria.
    9Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria.
    10Division of Gastroenterology and Hepatology, Department of Internal Medicine, Medical University of Graz, Graz, Austria.
    11Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
    12Department of Anesthesiology and Intensive Care, Experimental Research Center, University of Rostock, Rostock, Germany.
    13Department of Anesthesiology and Intensive Care, Experimental Research Center, University of Rostock, Rostock, Germany.
    14Department of Paediatric and Adolescent Surgery, Medical University of Graz, Graz, Austria.
    15Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
    Abstract

    Probiotics are generally considered as safe, but infections may rarely occur in vulnerable patients. Alternatives to live microorganisms to manage dysbiosis may be of interest in these patients. Reuterin is a complex component system exhibiting broad spectrum antimicrobial activity and a possible candidate substance in these cases. Reuterin supernatant was cultured from in a bioreactor in a two-step process. Storage stability at -20°C and effect of repeated freeze-thaw cycles were assessed by high performance liquid chromatography (HPLC). Antimicrobial activity was tested against , , , , , , , , and . Male BALBc mice were gavage fed with reuterin supernatant ( = 10) or culture medium ( = 10). Fecal volatile organic compounds (VOC) were assessed by gas chromatography mass spectroscopy; the microbiome was examined by 16S rRNA gene sequencing. The supernatant contained 13.4 g/L reuterin (3-hydroxypropionaldehyde; 3-HPA). 3-HPA content remained stable at -20°C for 35 days followed by a slow decrease of its concentration. Repeated freezing/thawing caused a slow 3-HPA decrease. Antimicrobial activity was encountered against , , and . Microbiome analysis showed no differences in alpha and beta diversity markers. Linear discriminant effect size (LEfSe) analysis identified and (in the reuterin medium group) and , , , and (in the reuterin group) as markers for group differentiation. VOC analysis showed a significant decrease of heptane and increase of 3-methylbutanal in the reuterin group. The supernatant produced in this study contained acceptable amounts of 3-HPA remaining stable for 35 days at -20°C and exhibiting an antimicrobial effect against , , and . Under conditions, the reuterin supernatant caused alterations of the fecal microbiome. In the fecal, VOC analysis decreased heptane and increased 3-methylbutanal were encountered. These findings suggest the high potential of the reuterin system to influence the intestinal microbiome in health and disease, which needs to be examined in detail in future projects.


    Copyright © 2021 Castellani, Obermüller, Kienesberger, Singer, Peterbauer, Grabherr, Mayrhofer, Klymiuk, Horvath, Stadlbauer, Russmayer, Miekisch, Fuchs, Till and Heinl.

    KEYWORDS: 3-hydroxypropionaldehyde, antimicrobial activity, microbiome, postbiotics, reuterin, volatile organic compound

    Publikations ID: 34394042
    Quelle: öffnen
     
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