Abk�rzung zur Hauptnavigation Abk�rzung zu den Newsmeldungen Abk�rzung zu den Topstories  
  Barrierefreiheit    Kontakt MedUni Wien    Intranet    MedUni Wien - Shop    Universitätsbibliothek    Universitätsklinikum AKH Wien  
 
ccc_logo_en.gif
 
AKH Wien
 
 
Hauptnavigation
  • Livestream 2021
  • Home
  • Über das CCC
    • Allgemeines
    • Leitung der Organisationseinheit
    • CCC-Office Team
    • Kliniken und Partner
    • Qualitätsmanagement
    • Kontakt
  • PatientInnen
    • Covid-19
    • Allgemeines
    • Cancer School
    • Terminvereinbarung
    • Pflegeambulanz
    • PatientInnenvertretung
    • Links
  • Klinischer Bereich
    • Allgemeines
    • CCC Tumorboards
  • Wissenschaft & Forschung
    • Young CCC
    • CCC-ExpertInnenvideos
    • CCC Forschungscluster
    • CCC Units
    • CCC Platforms
    • Translationale Forschung
    • CCC Best Paper Award
    • CCC-TRIO Symposium
    • Kontakt/Links
  • Lehre
    • CCC Cancer School
    • Vienna International Summer School on Clinical and Experimental Oncology - VSSO
    • CCC Excellence Lecture
    • Interdisziplinäre onkologische Ausbildung
    • Klinisch-Praktisches Jahr (KPJ)
    • PhD Programme
    • Postgraduelle Fort- und Weiterbildung
    • Information/Contact
 
 
Subnavigation
    Inhaltsbereich


    Zurück zur Übersicht
    The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation. 2020 Mar 30. pii: S1053-2498(20)30802-0. doi: 10.1016/j.healun.2020.01.785
    Molecular Assessment of Single-Piece Mucosal Biopsies is Highly Reproducible Compared to Transbronchial Biopsies in Lung Transplantation.
    Parkes MD1,  Halloran PF2,  Timofte IL3,  Snell GI4,  Westall GP5,  Roux A6,  Havlin J7,  Hachem R8,  Kreisel D9,  Levine D10,  Juvet S11,  Keshavjee S12,  Jaksch P13,  Klepetko W14,  Hirji A15,  Weinkauf J16,  Halloran KM17
    Author information
    1University of Alberta, Edmonton, AB, Canada.
    2University of Alberta, Edmonton, AB, Canada.
    3University of Maryland, Baltimore, MD.
    4Alfred Hospital Lung Transplant Service, Melbourne, Australia.
    5Alfred Hospital Lung Transplant Service, Melbourne, Australia.
    6Hôpital Foch, Paris, France.
    7University Hospital Motol, Prague, Czech Republic.
    8Washington University in St. Louis, St. Louis, MO.
    9Washington University in St. Louis, St. Louis, MO.
    10University of Texas San Antonio, San Antonio, TX.
    11Toronto Lung Transplant Program, University Health Network, Toronto, ON, Canada.
    12Toronto Lung Transplant Program, University Health Network, Toronto, ON, Canada.
    13Medical University of Vienna, Vienna, Austria.
    14Medical University of Vienna, Vienna, Austria.
    15University of Alberta, Edmonton, AB, Canada.
    16University of Alberta, Edmonton, AB, Canada.
    17University of Alberta, Edmonton, AB, Canada.
    Abstract

    PURPOSE: Histologic assessment for rejection on transbronchial biopsies (TBB) has poor reproducibility. Mucosal biopsies are safer than TBBs but their histologic assessment has not been systematically studied or standardized. We developed molecular platforms to assess TBBs and mucosal biopsies from the third airway bifurcation (3BMB). We examined the reproducibility of scores assigned by molecular assessment of both biopsy types in lung transplant patients from 9 international centers.

    METHODS: We trained machine learning models (archetypal analysis, principal component analysis) on microarray data from 457 TBBs and 243 3BMBs. We processed 14 pairs of TBB pieces (28 total) taken during the same procedure and 13 pairs of 3BMBs (26 total). The models assigned each biopsy sets of archetype scores (denoted by "R") and principal component scores (denoted by "PC") describing injury and rejection phenotypes. We studied within-score agreement between biopsy pairs using intraclass correlations (ICC).

    RESULTS: Most scores had excellent reproducibility in 3BMB pairs compared to TBBs (Table 1). In 3BMBs the R1 (normal), R2 (rejection), R3 (late injury), PC1 (inflammation/rejection), and PC2 (late injury) scores were highly reproducible, but R4 and PC3 (both recent injury) were not. In TBBs the R2 (rejection) and PC1 (inflammation/rejection) scores were moderately reproducible; other scores had poor reproducibility.

    CONCLUSION: Molecular assessment of paired, single piece 3BMBs achieves excellent reproducibility in its key scores. TBB scores were less reproducible likely because of tissue heterogeneity and greater anatomic distance between samples. Measurements could be stabilized by combining ≥2 pieces per microarray. Importantly, molecular assessment with 3BMBs or TBBs requires fewer pieces (2-3) than TBB histology, which requires up to ten. These data support continued study of the diagnostic and prognostic utility of 3BMBs in lung transplantation.


    Copyright © 2020. Published by Elsevier Inc.

    Publikations ID: 32465032
    Quelle: öffnen
     
    Drucken
     
    ccc_logo_en.gif
    ccc_logo_en.gif
    ccc_logo_en.gif

    Schnellinfo

     
    -- Initiative Krebsforschung / Krebsforschungslauf

    -- Cancer Care
    -- Kliniken und Partner
    -- CCC Cancer School
    -- Young CCC
    -- CCC Tumorboards
    -- CCC Forschungscluster
    -- CCC Units
    -- CCC Platforms
    -- SOPs / Leitlinien
    -- Kontakt
    Zuklappen
     
    Ausklappen
     
     

    Featured

     
     
     
     
     
     
     
     
     
     
     
     
     
    © MedUni Wien |
     Impressum | Nutzungsbedingungen | Kontakt