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| Molecular cell. 2019 Dec 4. pii: S1097-2765(19)30840-8. doi: 10.1016/j.molcel.2019.11.011 |
| Polycomb Group Proteins Regulate Chromatin Architecture in Mouse Oocytes and Early Embryos. |
| Du Z1, Zheng H2, Kawamura YK3, Zhang K4, Gassler J5, Powell S6, Xu Q7, Lin Z8, Xu K9, Zhou Q10, Ozonov EA11, Véron N12, Huang B13, Li L14, Yu G15, Liu L16, Au Yeung WK17, Wang P18, Chang L19, Wang Q20, He A21, Sun Y22, Na J23, Sun Q24, Sasaki H25, Tachibana K26, Peters AHFM27, Xie W28 |
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Abstract In mammals, chromatin organization undergoes drastic reorganization during oocyte development. However, the dynamics of three-dimensional chromatin structure in this process is poorly characterized. Using low-input Hi-C (genome-wide chromatin conformation capture), we found that a unique chromatin organization gradually appears during mouse oocyte growth. Oocytes at late stages show self-interacting, cohesin-independent compartmental domains marked by H3K27me3, therefore termed Polycomb-associating domains (PADs). PADs and inter-PAD (iPAD) regions form compartment-like structures with strong inter-domain interactions among nearby PADs. PADs disassemble upon meiotic resumption from diplotene arrest but briefly reappear on the maternal genome after fertilization. Upon maternal depletion of Eed, PADs are largely intact in oocytes, but their reestablishment after fertilization is compromised. By contrast, depletion of Polycomb repressive complex 1 (PRC1) proteins attenuates PADs in oocytes, which is associated with substantial gene de-repression in PADs. These data reveal a critical role of Polycomb in regulating chromatin architecture during mammalian oocyte growth and early development. |
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Copyright © 2019 Elsevier Inc. All rights reserved. |
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KEYWORDS: Chromatin architecture, Embryos, Hi-C, Oocytes, Polycomb, Polycomb-associating domains (PADs) |
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Publikations ID: 31837995 Quelle: öffnen |
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