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    European journal of immunology. doi: 10.1002/eji.201970107
    Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).
    Cossarizza A1,  Chang HD2,  Radbruch A3,  Acs A4,  Adam D5,  Adam-Klages S6,  Agace WW7,  Aghaeepour N8,  Akdis M9,  Allez M10,  Almeida LN11,  Alvisi G12,  Anderson G13,  Andrä I14,  Annunziato F15,  Anselmo A16,  Bacher P17,  Baldari CT18,  Bari S19,  Barnaba V20,  Barros-Martins J21,  Battistini L22,  Bauer W23,  Baumgart S24,  Baumgarth N25,  Baumjohann D26,  Baying B27,  Bebawy M28,  Becher B29,  Beisker W30,  Benes V31,  Beyaert R32,  Blanco A33,  Boardman DA34,  Bogdan C35,  Borger JG36,  Borsellino G37,  Boulais PE38,  Bradford JA39,  Brenner D40,  Brinkman RR41,  Brooks AES42,  Busch DH43,  Büscher M44,  Bushnell TP45,  Calzetti F46,  Cameron G47,  Cammarata I48,  Cao X49,  Cardell SL50,  Casola S51,  Cassatella MA52,  Cavani A53,  Celada A54,  Chatenoud L55,  Chattopadhyay PK56,  Chow S57,  Christakou E58,  Čičin-Šain L59,  Clerici M60,  Colombo FS61,  Cook L62,  Cooke A63,  Cooper AM64,  Corbett AJ65,  Cosma A66,  Cosmi L67,  Coulie PG68,  Cumano A69,  Cvetkovic L70,  Dang VD71,  Dang-Heine C72,  Davey MS73,  Davies D74,  De Biasi S75,  Del Zotto G76,  Dela Cruz GV77,  Delacher M78,  Della Bella S79,  Dellabona P80,  Deniz G81,  Dessing M82,  Di Santo JP83,  Diefenbach A84,  Dieli F85,  Dolf A86,  Dörner T87,  Dress RJ88,  Dudziak D89,  Dustin M90,  Dutertre CA91,  Ebner F92,  Eckle SBG93,  Edinger M94,  Eede P95,  Ehrhardt GRA96,  Eich M97,  Engel P98,  Engelhardt B99,  Erdei A100,  Esser C101,  Everts B102,  Evrard M103,  Falk CS104,  Fehniger TA105,  Felipo-Benavent M106,  Ferry H107,  Feuerer M108,  Filby A109,  Filkor K110,  Fillatreau S111,  Follo M112,  Förster I113,  Foster J114,  Foulds GA115,  Frehse B116,  Frenette PS117,  Frischbutter S118,  Fritzsche W119,  Galbraith DW120,  Gangaev A121,  Garbi N122,  Gaudilliere B123,  Gazzinelli RT124,  Geginat J125,  Gerner W126,  Gherardin NA127,  Ghoreschi K128,  Gibellini L129,  Ginhoux F130,  Goda K131,  Godfrey DI132,  Goettlinger C133,  González-Navajas JM134,  Goodyear CS135,  Gori A136,  Grogan JL137,  Grummitt D138,  Grützkau A139,  Haftmann C140,  Hahn J141,  Hammad H142,  Hämmerling G143,  Hansmann L144,  Hansson G145,  Harpur CM146,  Hartmann S147,  Hauser A148,  Hauser AE149,  Haviland DL150,  Hedley D151,  Hernández DC152,  Herrera G153,  Herrmann M154,  Hess C155,  Höfer T156,  Hoffmann P157,  Hogquist K158,  Holland T159,  Höllt T160,  Holmdahl R161,  Hombrink P162,  Houston JP163,  Hoyer BF164,  Huang B165,  Huang FP166,  Huber JE167,  Huehn J168,  Hundemer M169,  Hunter CA170,  Hwang WYK171,  Iannone A172,  Ingelfinger F173,  Ivison SM174,  Jäck HM175,  Jani PK176,  Jávega B177,  Jonjic S178,  Kaiser T179,  Kalina T180,  Kamradt T181,  Kaufmann SHE182,  Keller B183,  Ketelaars SLC184,  Khalilnezhad A185,  Khan S186,  Kisielow J187,  Klenerman P188,  Knopf J189,  Koay HF190,  Kobow K191,  Kolls JK192,  Kong WT193,  Kopf M194,  Korn T195,  Kriegsmann K196,  Kristyanto H197,  Kroneis T198,  Krueger A199,  Kühne J200,  Kukat C201,  Kunkel D202,  Kunze-Schumacher H203,  Kurosaki T204,  Kurts C205,  Kvistborg P206,  Kwok I207,  Landry J208,  Lantz O209,  Lanuti P210,  LaRosa F211,  Lehuen A212,  LeibundGut-Landmann S213,  Leipold MD214,  Leung LYT215,  Levings MK216,  Lino AC217,  Liotta F218,  Litwin V219,  Liu Y220,  Ljunggren HG221,  Lohoff M222,  Lombardi G223,  Lopez L224,  López-Botet M225,  Lovett-Racke AE226,  Lubberts E227,  Luche H228,  Ludewig B229,  Lugli E230,  Lunemann S231,  Maecker HT232,  Maggi L233,  Maguire O234,  Mair F235,  Mair KH236,  Mantovani A237,  Manz RA238,  Marshall AJ239,  Martínez-Romero A240,  Martrus G241,  Marventano I242,  Maslinski W243,  Matarese G244,  Mattioli AV245,  Maueröder C246,  Mazzoni A247,  McCluskey J248,  McGrath M249,  McGuire HM250,  McInnes IB251,  Mei HE252,  Melchers F253,  Melzer S254,  Mielenz D255,  Miller SD256,  Mills KHG257,  Minderman H258,  Mjösberg J259,  Moore J260,  Moran B261,  Moretta L262,  Mosmann TR263,  Müller S264,  Multhoff G265,  Muñoz LE266,  Münz C267,  Nakayama T268,  Nasi M269,  Neumann K270,  Ng LG271,  Niedobitek A272,  Nourshargh S273,  Núñez G274,  O'Connor JE275,  Ochel A276,  Oja A277,  Ordonez D278,  Orfao A279,  Orlowski-Oliver E280,  Ouyang W281,  Oxenius A282,  Palankar R283,  Panse I284,  Pattanapanyasat K285,  Paulsen M286,  Pavlinic D287,  Penter L288,  Peterson P289,  Peth C290,  Petriz J291,  Piancone F292,  Pickl WF293,  Piconese S294,  Pinti M295,  Pockley AG296,  Podolska MJ297,  Poon Z298,  Pracht K299,  Prinz I300,  Pucillo CEM301,  Quataert SA302,  Quatrini L303,  Quinn KM304,  Radbruch H305,  Radstake TRDJ306,  Rahmig S307,  Rahn HP308,  Rajwa B309,  Ravichandran G310,  Raz Y311,  Rebhahn JA312,  Recktenwald D313,  Reimer D314,  Reis E Sousa C315,  Remmerswaal EBM316,  Richter L317,  Rico LG318,  Riddell A319,  Rieger AM320,  Robinson JP321,  Romagnani C322,  Rubartelli A323,  Ruland J324,  Saalmüller A325,  Saeys Y326,  Saito T327,  Sakaguchi S328,  Sala-de-Oyanguren F329,  Samstag Y330,  Sanderson S331,  Sandrock I332,  Santoni A333,  Sanz RB334,  Saresella M335,  Sautes-Fridman C336,  Sawitzki B337,  Schadt L338,  Scheffold A339,  Scherer HU340,  Schiemann M341,  Schildberg FA342,  Schimisky E343,  Schlitzer A344,  Schlosser J345,  Schmid S346,  Schmitt S347,  Schober K348,  Schraivogel D349,  Schuh W350,  Schüler T351,  Schulte R352,  Schulz AR353,  Schulz SR354,  Scottá C355,  Scott-Algara D356,  Sester DP357,  Shankey TV358,  Silva-Santos B359,  Simon AK360,  Sitnik KM361,  Sozzani S362,  Speiser DE363,  Spidlen J364,  Stahlberg A365,  Stall AM366,  Stanley N367,  Stark R368,  Stehle C369,  Steinmetz T370,  Stockinger H371,  Takahama Y372,  Takeda K373,  Tan L374,  Tárnok A375,  Tiegs G376,  Toldi G377,  Tornack J378,  Traggiai E379,  Trebak M380,  Tree TIM381,  Trotter J382,  Trowsdale J383,  Tsoumakidou M384,  Ulrich H385,  Urbanczyk S386,  van de Veen W387,  van den Broek M388,  van der Pol E389,  Van Gassen S390,  Van Isterdael G391,  van Lier RAW392,  Veldhoen M393,  Vento-Asturias S394,  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    Author information
    1Department of Medical and Surgical Sciences for Children and Adults, Univ. of Modena and Reggio Emilia School of Medicine, Modena, Italy.
    2Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    3Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    4Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
    5Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
    6Institut für Transfusionsmedizin, Universitätsklinik Schleswig-Holstein, Kiel, Germany.
    7Mucosal Immunology group, Department of Health Technology, Technical University of Denmark, Kgs. Lyngby, Denmark.
    8Departments of Anesthesiology, Pain and Perioperative Medicine, Biomedical Data Sciences; and Pediatrics, Stanford University, Stanford, CA, USA.
    9Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland.
    10Université de Paris, Institut de Recherche Saint-Louis, INSERM U1160, and Gastroenterology Department, Hôpital Saint-Louis - APHP, Paris, France.
    11Institute for Systemic Inflammation Research, University of Luebeck, Luebeck, Germany.
    12Laboratory of Translational Immunology, Humanitas Clinical and Research Center, Rozzano, Italy.
    13University of Birmingham, Birmingham, UK.
    14Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
    15Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
    16Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy.
    17Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
    18Department of Life Sciences, University of Siena, Siena, Italy.
    19Division of Medical Sciences, National Cancer Centre Singapore, Singapore.
    20Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Rome, Italy.
    21Institute of Immunology, Hannover Medical School, Hannover, Germany.
    22IRCCS Fondazione Santa Lucia, Rome, Italy.
    23Division of Immunology, Allergy and Infectious Diseases, Department of Dermatology, Medical University of Vienna, Vienna, Austria.
    24Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    25Center for Comparative Medicine & Dept. Pathology, Microbiology & Immunology, University of California, Davis, CA, USA.
    26Institute for Immunology, Faculty of Medicine, Biomedical Center, LMU Munich, Planegg-Martinsried, Germany.
    27Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
    28Discipline of Pharmacy, Graduate School of Health, The University of Technology Sydney, Sydney, NSW, Australia.
    29Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
    30Flow Cytometry Laboratory, Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München, German Research Center for Environmental Health, München, Germany.
    31Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
    32Department of Biomedical Molecular Biology, Center for Inflammation Research, Ghent University - VIB, Ghent, Belgium.
    33Flow Cytometry Core Technologies, UCD Conway Institute, University College Dublin, Dublin, Ireland.
    34Department of Surgery, The University of British Columbia, Vancouver, Canada.
    35Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Erlangen, Germany.
    36Department of Immunology and Pathology, Monash University, Melbourne, Victoria, Australia.
    37Neuroimmunology and Flow Cytometry Units, Fondazione Santa Lucia IRCCS, Rome, Italy.
    38Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA.
    39Thermo Fisher Scientific, Eugene, Oregon, USA.
    40Luxembourg Institute of Health, Department of Infection and Immunity, Experimental and Molecular Immunology, Esch-sur-Alzette, Luxembourg.
    41Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada.
    42University of Auckland, School of Biological Sciences, Maurice Wilkins Center, Auckland, New Zealand.
    43Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
    44Biophysics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
    45Department of Pediatrics and Shared Resource Laboratories, University of Rochester Medical Center, Rochester, NY, USA.
    46University of Verona, Department of Medicine, Section of General Pathology, Verona, Italy.
    47Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia.
    48Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Rome, Italy.
    49National Key Laboratory of Medical Immunology, Nankai University, Tianjin, China.
    50Department of Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden.
    51The FIRC Institute of Molecular Oncology (FOM), Milan, Italy.
    52University of Verona, Department of Medicine, Section of General Pathology, Verona, Italy.
    53National Institute for Health, Migration and Poverty (INMP), Rome, Italy.
    54Macrophage Biology Group, School of Biology, University of Barcelona, Barcelona, Spain.
    55Université Paris Descartes, Institut National de la Santé et de la Recherche Médicale, Paris, France.
    56Isaac and Laura Perlmutter Cancer Center, NYU-Langone Medical Center, New York, USA.
    57Divsion of Medical Oncology and Hematology, Princess Margaret Hospital, Toronto, Ontario, Canada.
    58Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, UK.
    59Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
    60IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.
    61Flow Cytometry Core, Humanitas Clinical and Research Center, Milan, Italy.
    62BC Children's Hospital Research Institute, Vancouver, Canada.
    63Department of Pathology, University of Cambridge, Cambridge, UK.
    64Department of Respiratory Sciences, University of Leicester, Leicester, UK.
    65Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia.
    66National Cytometry Platform, Luxembourg Institute of Health, Department of Infection and Immunity, Esch-sur-Alzette, Luxembourg.
    67Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
    68de Duve Institute, Université catholique de Louvain, Brussels, Belgium.
    69Unit Lymphopoiesis, Department of Immunology, Institut Pasteur, Paris, France.
    70Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
    71Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    72Clinical Research Unit, Berlin Institute of Health (BIH), Charite Universitätsmedizin Berlin, Berlin, Germany.
    73Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
    74Flow Cytometry Scientific Technology Platform, The Francis Crick Institute, London, UK.
    75Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.
    76IRCCS Istituto Giannina Gaslini, Genova, Italy.
    77Novo Nordisk Foundation Center for Stem Cell Biology - DanStem, University of Copenhagen, Copenhagen, Denmark.
    78Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany.
    79Department of Medical Biotechnologies and Translational Medicine, University of Milan, Milan, Italy.
    80Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, Milan, Italy.
    81Istanbul University, Aziz Sancar Institute of Experimental Medicine, Department of Immunology, Istanbul, Turkey.
    82Sony Europe Ltd, Weybridge, UK.
    83Innate Immunty Unit, Department of Immunology, Institut Pasteur, Paris, France.
    84Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    85University of Palermo, Central Laboratory of Advanced Diagnosis and Biomedical Research, Department of Biomedicine, Neurosciences and Advanced Diagnostics, Palermo, Italy.
    86Flow Cytometry Core Facility, Institute of Experimental Immunology, University of Bonn, Bonn, Germany.
    87Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    88Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
    89Department of Dermatology, Laboratory of Dendritic Cell Biology, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), University Hospital Erlangen, Erlangen, Germany.
    90Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK.
    91Program in Emerging Infectious Disease, Duke-NUS Medical School, Singapore.
    92Institute of Immunology, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany.
    93Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia.
    94Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany.
    95Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology, Germany.
    96Department of Immunology, University of Toronto, Toronto, ON, Canada.
    97Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH), Heidelberg, Germany.
    98University of Barcelona, Faculty of Medicine and Health Sciences, Department of Biomedical Sciences, Barcelona, Spain.
    99Theodor Kocher Institute, University of Bern, Bern, Switzerland.
    100Department of Immunology, University L. Eotvos, Budapest, Hungary.
    101Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany.
    102Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
    103Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
    104Institute of Transplant Immunology, Hannover Medical School, MHH, Hannover, Germany.
    105Division of Oncology, Washington University School of Medicine, St. Louis, MO, USA.
    106Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Principe Felipe Research Center, Valencia, Spain.
    107Experimental Medicine Division, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
    108Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany.
    109The Flow Cytometry Core Facility, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK.
    110MDQuest Ltd., Szeged, Hungary.
    111Institut Necker-Enfants Malades, Université Paris Descartes Sorbonne Paris Cité, Faculté de Médecine, AP-HP, Hôpital Necker Enfants Malades, INSERM U1151-CNRS UMR 8253, Paris, France.
    112Department of Medicine I, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
    113Immunology and Environment, LIMES Institute, University of Bonn, Bonn, Germany.
    114Owl Biomedical Inc., Santa Barbara, USA.
    115John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK.
    116Institute for Systemic Inflammation Research, University of Luebeck, Luebeck, Germany.
    117Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY, USA.
    118Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    119Nanobiophotonics Department, Leibniz Institute of Photonic Technology (IPHT), Jena, Germany.
    120School of Plant Sciences and Bio5 Institute, University of Arizona, Tucson, USA.
    121Division of Molecular Oncology and Immunology, the Netherlands Cancer Institute, Amsterdam, The Netherlands.
    122Institute of Experimental Immunology, University of Bonn, Germany.
    123Stanford Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, CA, USA.
    124Fundação Oswaldo Cruz - Minas, Laboratory of Immunopatology, Belo Horizonte, MG, Brazil.
    125INGM - Fondazione Istituto Nazionale di Genetica Molecolare "Ronmeo ed Enrica Invernizzi", Milan, Italy.
    126Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.
    127Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia.
    128Department of Dermatology, Venereology and Allergology, Charité - Universitätsmedizin Berlin, Berlin, Germany.
    129Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.
    130Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
    131Department of Bioengineering, University of California, Los Angeles, California, USA.
    132Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia.
    133Institut für Genetik, Universität zu Köln, Köln, Germany.
    134Alicante Institute for Health and Biomedical Research (ISABIAL), Alicante, Spain.
    135Institute of Infection Immunity and Inflammation, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, UK.
    136Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, University of Milan.
    137Cancer Immunology Research, Genentech, South San Francisco, CA, USA.
    138Owl Biomedical Inc., Santa Barbara, USA.
    139Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    140Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
    141Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen.
    142Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Zwijnaarde, Belgium.
    143German Cancer Research Center (DKFZ), Heidelberg, Germany.
    144Berlin Institute of Health (BIH), Berlin, Germany.
    145Department of Medicine and Center for Molecular Medicine at Karolinska University Hospital, Solna, Sweden.
    146Murdoch Children's Research Institute, Parkville, Victoria, Australia.
    147Institute of Immunology, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany.
    148Department of Internal Medicine III, University Hospital Regensburg, Germany.
    149Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    150Flow Cytometry, Houston Methodist Hospital Research Institute, Houston, TX, USA.
    151Divsion of Medical Oncology and Hematology, Princess Margaret Hospital, Toronto, Ontario, Canada.
    152Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    153Cytometry Service, Incliva Foundation. Clinic Hospital and Faculty of Medicine, University of Valencia, Valencia, Spain.
    154Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen.
    155Immunobiology Laboratory, Department of Biomedicine, University and University Hospital Basel, Basel, Switzerland.
    156German Cancer Research Center (DKFZ), Division of Theoretical Systems Biology, Heidelberg, Germany.
    157Regensburg Center for Interventional Immunology (RCI), Regensburg, Germany.
    158Center for Immunology, University of Minnesota, Minneapolis, MN, USA.
    159Institute of Experimental Immunology, University of Bonn, Germany.
    160Leiden Computational Biology Center, Leiden University Medical Center, Leiden, The Netherlands.
    161Karolinska Institutet Biomedicum, Solna, Sweden.
    162Department of Experimental Immunology, Amsterdam Infection and Immunity Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
    163Department of Chemical & Materials Engineering, New Mexico State University, Las Cruces, NM, USA.
    164Rheumatologie/Klinische Immunologie, Klinik für Innere Medizin I und Exzellenzzentrum Entzündungsmedizin, Universitätsklinikum Schleswig-Holstein, Kiel, Germany.
    165Department of Immunology & National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing, China.
    166Institute for Advanced Study (IAS), Shenzhen University, Shenzhen, China.
    167Institute for Immunology, Faculty of Medicine, Biomedical Center, LMU Munich, Planegg-Martinsried, Germany.
    168Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
    169Department of Hematology, Oncology and Rheumatology, University Heidelberg, Heidelberg, Germany.
    170Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA.
    171Department of Hematology, Singapore General Hospital, Singapore.
    172Department of Diagnostic Medicine, Clinical and Public Health, Univ. of Modena and Reggio Emilia, Modena, Italy.
    173Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
    174Department of Surgery, The University of British Columbia, Vancouver, Canada.
    175Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
    176Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    177Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain.
    178Department of Histology and Embryology/Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia.
    179Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    180Department of Paediatric Haematology and Oncology, Second Faculty of Medicine, Charles University, Prague, Czech Republic.
    181Jena University Hospital, Institute of Immunology, Jena, Germany.
    182Max Planck Institute for Infection Biology, Berlin, Germany.
    183Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
    184Division of Molecular Oncology and Immunology, the Netherlands Cancer Institute, Amsterdam, The Netherlands.
    185Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
    186Department of Immunology, University of Toronto, Toronto, ON, Canada.
    187Institute of Molecular Health Sciences, ETH Zurich, Zürich, Switzerland.
    188Experimental Medicine Division, Nuffield Department of Medicine, University of Oxford, Oxford, UK.
    189Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen.
    190Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia.
    191Department of Neuropathology, Universitätsklinikum Erlangen, Germany.
    192John W Deming Endowed Chair in Internal Medicine, Center for Translational Research in Infection and Inflammation Tulane School of Medicine, New Orleans, LA, USA.
    193Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
    194Institute of Molecular Health Sciences, ETH Zurich, Zürich, Switzerland.
    195Department of Neurology, Technical University of Munich, Munich, Germany.
    196Department of Hematology, Oncology and Rheumatology, University Heidelberg, Heidelberg, Germany.
    197Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.
    198Division of Cell Biology, Histology & Embryology, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria.
    199Institute for Molecular Medicine, Goethe University Frankfurt, Frankfurt am Main, Germany.
    200Institute of Transplant Immunology, Hannover Medical School, MHH, Hannover, Germany.
    201FACS & Imaging Core Facility, Max Planck Institute for Biology of Ageing, Cologne, Germany.
    202Flow & Mass Cytometry Core Facility, Charité - Universitätsmedizin Berlin and Berlin Institute of Health, Berlin, Germany.
    203Institute for Molecular Medicine, Goethe University Frankfurt, Frankfurt am Main, Germany.
    204WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan.
    205Institute of Experimental Immunology, University of Bonn, Germany.
    206Division of Molecular Oncology and Immunology, the Netherlands Cancer Institute, Amsterdam, The Netherlands.
    207Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
    208Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
    209INSERM U932, PSL University, Institut Curie, Paris, France.
    210Department of Medicine and Aging Sciences, Centre on Aging Sciences and Translational Medicine (Ce.S.I.-Me.T.), University "G. d'Annunzio" of Chieti-Pescara, Chieti, Italy.
    211IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.
    212Institut Cochin, CNRS8104, INSERM1016, Department of Endocrinology, Metabolism and Diabetes, Université de Paris, Paris, France.
    213Section of Immunology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
    214The Human Immune Monitoring Center (HIMC), Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, CA, USA.
    215Department of Immunology, University of Toronto, Toronto, ON, Canada.
    216Department of Surgery, The University of British Columbia, Vancouver, Canada.
    217Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    218Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
    219Caprion Biosciences, ImmuneCarta, Montréal, Quebec, Canada.
    220Department of Immunology, University of Toronto, Toronto, ON, Canada.
    221Center for Infectious Medicine, Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, Stockholm, Sweden.
    222Inst. f. Med. Mikrobiology and Hospital Hygiene, University of Marburg, Germany.
    223King's College London, "Peter Gorer" Department of Immunobiology, London, UK.
    224Beckman Coulter, Inc, Miami, FL, USA.
    225IMIM(Hospital de Mar Medical Research Institute), University Pompeu Fabra, Barcelona, Spain.
    226Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH, USA.
    227Department of Rheumatology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
    228Centre d'Immunophénomique - CIPHE (PHENOMIN), Aix Marseille Université (UMS3367), Inserm (US012), CNRS (UMS3367), Marseille, France.
    229Institute of Immunobiology, Kantonsspital St.Gallen, St. Gallen, Switzerland.
    230Laboratory of Translational Immunology, Humanitas Clinical and Research Center, Rozzano, Italy.
    231Department of Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
    232Institute for Immunity, Transplantation, and Infection, Stanford University School of Medicine, Stanford, CA, USA.
    233Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
    234Flow and Image Cytometry Shared Resource, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.
    235Fred Hutchinson Cancer Research Center, Vaccine and Infectious Disease Division, Seattle, WA, USA.
    236Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.
    237Istituto Clinico Humanitas IRCCS and Humanitas University, Pieve Emanuele, Milan, Italy.
    238Institute for Systemic Inflammation Research, University of Luebeck, Luebeck, Germany.
    239Department of Immunology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada.
    240Cytomics Technological Service, Principe Felipe Research Center, Valencia, Spain.
    241Department of Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
    242IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.
    243National Institute of Geriatrics, Rheumatology and Rehabilitation, Department of Pathophysiology and Immunology, Warsaw, Poland.
    244Treg Cell Lab, Dipartimento di Medicina Molecolare e Biotecologie Mediche, Università di Napoli Federico II and Istituto per l'Endocrinologia e l'Oncologia Sperimentale, Consiglio Nazionale delle Ricerche (IEOS-CNR), Napoli, Italy.
    245Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.
    246Cell Clearance in Health and Disease Lab, VIB Center for Inflammation Research, Ghent, Belgium.
    247Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
    248Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Parkville, Victoria, Australia.
    249Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    250Ramaciotti Facility for Human Systems Biology, and Discipline of Pathology, The University of Sydney, Camperdown, Australia.
    251Institute of Infection Immunity and Inflammation, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, UK.
    252Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    253Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    254Clinical Trial Center Leipzig, University Leipzig, Leipzig, Germany.
    255Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
    256Interdepartmental Immunobiology Center, Dept. of Microbiology-Immunology, Northwestern Univ. Medical School, Chicago, IL, USA.
    257Trinity College Dublin, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Dublin, Ireland.
    258Flow and Image Cytometry Shared Resource, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.
    259Center for Infectious Medicine, Department of Medicine Huddinge, ANA Futura, Karolinska Institutet, Stockholm, Sweden.
    260Abramson Cancer Center Flow Cytometry and Cell Sorting Shared Resource, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.
    261Trinity College Dublin, School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Dublin, Ireland.
    262Department of Immunology, IRCCS Bambino Gesu Children's Hospital, Rome, Italy.
    263David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
    264Centre for Environmental Research - UFZ, Department Environmental Microbiology, Leipzig, Germany.
    265Institute for Innovative Radiotherapy (iRT), Experimental Immune Biology, Helmholtz Zentrum München, Neuherberg, Germany.
    266Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen.
    267Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
    268Department of Immunology, Graduate School of Medicine, Chiba University, Chiba city, Chiba, Japan.
    269Department of Surgery, Medicine, Dentistry and Morphological Sciences, Univ. of Modena and Reggio Emilia, Modena, Italy.
    270Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
    271Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
    272Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    273Barts and The London School of Medicine and Dentistry, Queen Mary University of London, UK.
    274Department of Pathology and Rogel Cancer Center, the University of Michigan, Ann Arbor, Michigan, USA.
    275Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain.
    276Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
    277Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
    278Flow Cytometry Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
    279Department of Medicine, Cancer Research Centre (IBMCC-CSIC/USAL), Cytometry Service, University of Salamanca, CIBERONC and Institute for Biomedical Research of Salamanca (IBSAL), Salamanca, Spain.
    280Burnet Institute, AMREP Flow Cytometry Core Facility, Melbourne, Victoria, Australia.
    281Inflammation and Oncology, Research, Amgen Inc, South San Francisco, USA.
    282ETH Zurich, Institute of Microbiology, Zurich, Switzerland.
    283Department of Transfusion Medicine, Institute of Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany.
    284Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    285Center of Excellence for Flow Cytometry, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
    286Flow Cytometry Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
    287Genomics Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
    288Department of Hematology, Oncology, and Tumor Immunology, Charité - Universitätsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany.
    289Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia.
    290Biophysics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
    291Functional Cytomics Group, Josep Carreras Leukaemia Research Institute, Campus ICO-Germans Trias i Pujol, Universitat Autònoma de Barcelona, UAB, Badalona, Spain.
    292IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.
    293Institute of Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
    294Dipartimento di Medicina Interna e Specialità Mediche, Sapienza Università di Roma, Rome, Italy.
    295Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
    296John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK.
    297Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Department of Medicine 3, Rheumatology and Immunology, Universitätsklinikum Erlangen, Erlangen.
    298Department of Hematology, Singapore General Hospital, Singapore.
    299Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
    300Institute of Immunology, Hannover Medical School, Hannover, Germany.
    301Department of Immunology, University of Udine, Udine, Italy.
    302David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
    303Department of Immunology, IRCCS Bambino Gesu Children's Hospital, Rome, Italy.
    304School of Biomedical and Health Sciences, RMIT University, Bundoora, Victoria, Australia.
    305Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Neuropathology, Germany.
    306Department of Rheumatology and Clinical Immunology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands.
    307Regeneration in Hematopoiesis, Leibniz-Institute on Aging, Fritz-Lipmann-Institute (FLI), Jena, Germany.
    308Preparative Flow Cytometry, Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany.
    309Bindley Biosciences Center, Purdue University, West Lafayette, IN, USA.
    310Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
    311Department of Internal Medicine, Groene Hart Hospital, Gouda, The Netherlands.
    312David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
    313Desatoya LLC, Reno, NV, USA.
    314Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
    315Immunobiology Laboratory, The Francis Crick Institute, London, UK.
    316Department of Experimental Immunology, Amsterdam Infection and Immunity Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
    317Core Facility Flow Cytometry, Biomedical Center, Ludwig-Maximilians-University Munich, Germany.
    318Functional Cytomics Group, Josep Carreras Leukaemia Research Institute, Campus ICO-Germans Trias i Pujol, Universitat Autònoma de Barcelona, UAB, Badalona, Spain.
    319Flow Cytometry Scientific Technology Platform, The Francis Crick Institute, London, UK.
    320Department of Medical Microbiology and Immunology, University of Alberta, Alberta, Canada.
    321Purdue University Cytometry Laboratories, Purdue University, West Lafayette, IN, USA.
    322Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    323Cell Biology Unit, IRCCS Ospedale Policlinico San Martino, Genova, Italy.
    324Institut für Klinische Chemie und Pathobiochemie, Fakultät für Medizin, Technische Universität München, München, Germany.
    325Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria.
    326Data Mining and Modeling for Biomedicine, VIB-UGent Center for Inflammation Research, Ghent, Belgium.
    327RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.
    328WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan.
    329Flow Cytometry Facility, Ludwig Cancer Institute, Faculty of Medicine and Biology, University of Lausanne, Epalinges, Switzerland.
    330Heidelberg University, Institute of Immunology, Section of Molecular Immunology, Heidelberg, Germany.
    331Translational Immunology Laboratory, NIHR BRC, University of Oxford, Kennedy Institute of Rheumatology, Oxford, UK.
    332Institute of Immunology, Hannover Medical School, Hannover, Germany.
    333Department of Molecular Medicine, Sapienza University of Rome, IRCCS, Neuromed, Pozzilli, Italy.
    334Institute of Transplant Immunology, Hannover Medical School, MHH, Hannover, Germany.
    335IRCCS Fondazione Don Carlo Gnocchi, Milan, Italy.
    336Centre de Recherches des Cordeliers, Equipe Cancer et Immunité anti-tumorale, Paris, France.
    337Charité - Universitätsmedizin Berlin, and Berlin Institute of Health, Institute of Medical Immunology, Berlin, Germany.
    338Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
    339Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
    340Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands.
    341Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
    342Clinic for Orthopedics and Trauma Surgery, University Hospital Bonn, Bonn, Germany.
    343R&D Reagents, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
    344Quantitative Systems Biology, Life & Medical Sciences Institute, University of Bonn, Bonn, Germany.
    345Institute of Immunology, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany.
    346Internal Medicine I, University Hospital Regensburg, Germany.
    347Flow Cytometry Core Facility, German Cancer Research Centre (DKFZ), Heidelberg, Germany.
    348Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, Technische Universität München, Munich, Germany.
    349Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
    350Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
    351Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, Magdeburg, Germany.
    352University of Cambridge, Cambridge Institute for Medical Research, Cambridge, UK.
    353Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    354Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
    355King's College London, "Peter Gorer" Department of Immunobiology, London, UK.
    356Institut Pasteur, Cellular Lymphocytes Biology, Immunology Departement, Paris, France.
    357TRI Flow Cytometry Suite (TRI.fcs), Translational Research Institute, Wooloongabba, QLD, Australia.
    358AsedaSciences, West Lafayette, IN, USA.
    359Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Portugal.
    360Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK.
    361Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
    362Dept. Molecular Translational Medicine, University of Brescia, Brescia, Italy.
    363Department of Oncology, University of Lausanne and CHUV, Epalinges, Switzerland.
    364BD Life Sciences, Ashland, OR, USA.
    365Lundberg Laboratory for Cancer, Department of Pathology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden.
    366BD Life Sciences, San Diego, CA, USA.
    367Departments of Anesthesiology, Pain and Perioperative Medicine, Biomedical Data Sciences; and Pediatrics, Stanford University, Stanford, CA, USA.
    368Department of Experimental Immunology, Amsterdam Infection and Immunity Institute, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
    369Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    370Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
    371Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
    372National Institutes of Health, Bethesda, MD, USA.
    373WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan.
    374Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
    375Departement for Therapy Validation, Fraunhofer Institute for Cell Therapy and Immunology IZI, Leipzig, Germany.
    376Institute of Experimental Immunology and Hepatology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
    377MDQuest Ltd., Szeged, Hungary.
    378Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    379Novartis Biologics Center, Mechanistic Immunology Unit, Novartis Institute for Biomedical Research, NIBR, Basel, Switzerland.
    380Department of Cellular and Molecular Physiology, Penn State University College of Medicine, PA, United States.
    381Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, UK.
    382BD Life Sciences, San Diego, CA, USA.
    383Department of Pathology, University of Cambridge, Cambridge, UK.
    384Institute of Bioinnovation, BSRC Alexander Fleming, Vari, Greece.
    385Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP, Brazil.
    386Division of Molecular Immunology, Nikolaus-Fiebiger-Center, Dept. of Internal Medicine III, University of Erlangen-Nuremberg, Erlangen, Germany.
    387Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland.
    388Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland.
    389Vesicle Observation Center, Biomedical Engineering & Physics, Laboratory Experimental Clinical Chemistry, Amsterdam University Medical Centers, Location AMC, The Netherlands.
    390Data Mining and Modeling for Biomedicine, VIB-UGent Center for Inflammation Research, Ghent, Belgium.
    391VIB Flow Core, VIB Center for Inflammation Research, Ghent, Belgium.
    392Department of Hematopoiesis, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
    393Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Portugal.
    394Institute of Experimental Immunology, University of Bonn, Germany.
    395Unit Lymphopoiesis, Department of Immunology, Institut Pasteur, Paris, France.
    396Department of Infection Biology, University Hospital Erlangen, Friedrich-Alexander University Erlangen-Nuremberg (FAU), Erlangen, Germany.
    397BIH Center for Regenerative Therapies (BCRT) Charité Universitätsmedizin Berlin and Berlin Institute of Health, Core Unit ImmunoCheck.
    398Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
    399A·P·E-Applied Physics & Electronics, Berlin, Germany.
    400Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany.
    401Flow Cytometry Facility, Babraham Institute, Cambridge, UK.
    402Roswell Park Comprehensive Cancer Center, Elm and Carlton Streets, Buffalo, NY, USA.
    403Dept of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
    404The Scientific Platforms, the Westmead Institute for Medical Research, the Westmead Research Hub, Westmead, New South Wales, Australia.
    405Thermo Fisher Scientific, Eugene, Oregon, USA.
    406Department of Surgery, The University of British Columbia, Vancouver, Canada.
    407Department of Rheumatology and Clinical Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
    408Flow Cytometry Core Facility, Blizard Institute, Queen Mary London University, London, UK.
    409BCRT Flow Cytometry Lab, Berlin-Brandenburg Center for Regenerative Therapies, Charité - Universitätsmedizin Berlin.
    410Regeneration in Hematopoiesis, Leibniz-Institute on Aging, Fritz-Lipmann-Institute (FLI), Jena, Germany.
    411Medinfomatics Ltd., West Drayton, Middlesex, UK.
    412Department for Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund (IfADo), Dortmund, Germany.
    413Biophysics, R&D Engineering, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany.
    414Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
    415Deutsches Rheuma-Forschungszentrum (DRFZ), an Institute of the Leibniz Association, Berlin, Germany.
    416Institute for Cellular & Molecular Immunology, University Medical Center Göttingen, Göttingen, Germany.
    417Institute of Immunology, Hannover Medical School, Hannover, Germany.
    418Department of Infectious Disease, Imperial College London, UK.
    419Cooperation Unit for Experimental and Translational Cancer Immunology, Institute of Immunology (Charité - Universitätsmedizin Berlin) and German Cancer Research Center (DKFZ), Berlin, Germany.
    420WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan.
    421Institut für Immunologie, Christian-Albrechts-Universität zu Kiel, Kiel, Germany.
    422Department of Biology, Nikolaus-Fiebiger-Center for Molecular Medicine, Friedrich-Alexander-University Erlangen-Nuremberg, Erlangen, Germany.
    423Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland.
    424Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, Singapore.
    425University Bonn, Medical Faculty, Bonn, Germany.
    426Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, UK.
    427Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, Germany.
    428Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.
    429BD Biosciences, San Jose, CA, USA.
    430School of Computing Science, Simon Fraser University, Burnaby, Canada.
    431Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK.
    432Department of Rheumatology and Immunology, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
    433Department of Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
    434German Center for Infection Research (DZIF), Munich, Germany.
    435Maurice Müller Laboratories (Department of Biomedical Research), Universitätsklinik für Viszerale Chirurgie und Medizin Inselspital, University of Bern, Bern, Switzerland.
    436Max Planck Institute for Infection Biology, Berlin, Germany.
    Abstract

    These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.


    © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

    Publikations ID: 31633216
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