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| Frontiers in physiology. 2017 Nov 17. doi: 10.3389/fphys.2017.00839. pmc: PMC5698270 |
| LETM1-Mediated K+ and Na+ Homeostasis Regulates Mitochondrial Ca2+ Efflux. |
| Austin S1, Tavakoli M2, Pfeiffer C3, Seifert J4, Mattarei A5, De Stefani D6, Zoratti M7, Nowikovsky K8 |
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Abstract HIGHLIGHTS Monovalent cation homeostasis is dysregulated upon LETM1 depletionK+/H+ exchange activity is decreased in LETM1 knockdown cellsLETM1 depletion results in K+ accumulation in the mitochondrial matrixLETM1 knockdown does not affect expression of major mitochondrial Ca2+ transport modulatorsLETM1-regulated mitochondrial Ca2+ fluxes are dependent on Na+ Ca2+ transport across the inner membrane of mitochondria (IMM) is of major importance for their functions in bioenergetics, cell death and signaling. It is therefore tightly regulated. It has been recently proposed that LETM1-an IMM protein with a crucial role in mitochondrial K+/H+ exchange and volume homeostasis-also acts as a Ca2+/H+ exchanger. Here we show for the first time that lowering LETM1 gene expression by shRNA hampers mitochondrial K+/H+ and Na+/H+ exchange. Decreased exchange activity resulted in matrix K+ accumulation in these mitochondria. Furthermore, LETM1 depletion selectively decreased Na+/Ca2+ exchange mediated by NCLX, as observed in the presence of ruthenium red, a blocker of the Mitochondrial Ca2+ Uniporter (MCU). These data confirm a key role of LETM1 in monovalent cation homeostasis, and suggest that the effects of its modulation on mitochondrial transmembrane Ca2+ fluxes may reflect those on Na+/H+ exchange activity. |
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KEYWORDS: LETM1, calcium, mitochondrial cation/proton exchange, mitochondrial volume homeostasis, potassium, sodium |
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Publikations ID: 29204122 Quelle: öffnen |
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