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| eLife. 2014 May 27. doi: 10.7554/eLife.02009. pmc: PMC4070021 |
| Transport of soluble proteins through the Golgi occurs by diffusion via continuities across cisternae. |
| Beznoussenko GV1, Parashuraman S2, Rizzo R3, Polishchuk R4, Martella O5, Di Giandomenico D6, Fusella A7, Spaar A8, Sallese M9, Capestrano MG10, Pavelka M11, Vos MR12, Rikers YG13, Helms V14, Mironov AA15, Luini A16 |
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Abstract The mechanism of transport through the Golgi complex is not completely understood, insofar as no single transport mechanism appears to account for all of the observations. Here, we compare the transport of soluble secretory proteins (albumin and α1-antitrypsin) with that of supramolecular cargoes (e.g., procollagen) that are proposed to traverse the Golgi by compartment progression-maturation. We show that these soluble proteins traverse the Golgi much faster than procollagen while moving through the same stack. Moreover, we present kinetic and morphological observations that indicate that albumin transport occurs by diffusion via intercisternal continuities. These data provide evidence for a transport mechanism that applies to a major class of secretory proteins and indicate the co-existence of multiple intra-Golgi trafficking modes. |
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KEYWORDS: albumin, cell biology, golgi complex, human, intracellular trafficking, membrane tubules, soluble cargo proteins |
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Publikations ID: 24867214 Quelle: öffnen |
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